Recently, Agricultural Knowledge and Innovation Systems (AKISs) have gained considerable attention in scientific and political forums in the European Union (EU). AKIS is considered a key concept in identifying, analysing and assessing the various actors in the agricultural sector as well as their communication and interaction for innovation processes. Using qualitative expert interviews and organizational mapping, the features of national AKISs were investigated in selected EU member states (Belgium, France, Ireland, Germany, Portugal and the UK).