The objective of this paper is to analyse the olive oil value chain (OVC) in the Governorate of Medenine (south-east of Tunisia) and the relationships between its main operators for an effective involvement and better performance and resilience of olive sector. Based on semi structured interviews and participatory multi-stakeholders’ workshops, OVC has been analysed and described. MACTOR approach has been applied to establish linkages among chain operators and activities in a partnership approach.